Politecnico di Milano
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Articular Joints

Microfluidic platforms recreating the articular joint environment, combining cartilage, bone, and synovial tissues to study osteoarthritis, rheumatoid arthritis, and evaluate potential therapeutic interventions.

Joint-on-ChipCartilageOsteoarthritis
1

Osteoarthritis on Chip

We develop joint-on-chip models that recapitulate the hallmarks of osteoarthritis, including cartilage degradation, subchondral bone remodeling, and synovial inflammation. Using mechanically stimulated osteochondral microtissues, we study disease progression and evaluate disease-modifying drugs in a controlled microfluidic environment.

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Osteoarthritis on Chip — image coming soon

2

Developmental Biology

Our platforms model the mesenchymal condensation and chondrogenic differentiation processes that drive cartilage and bone development. By recapitulating morphogenetic gradients in microfluidic devices, we investigate the signaling pathways governing skeletal tissue formation and regeneration.

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Developmental Biology — image coming soon

3

Shear Stresses

Mechanical loading is a critical regulator of joint tissue homeostasis. We engineer microfluidic systems that apply controlled compressive and shear forces to cartilage constructs, enabling the study of mechanotransduction pathways and their role in both healthy maintenance and pathological degradation.

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Shear Stresses — image coming soon

4

Joint-on-Chip

Our integrated joint-on-chip platform combines cartilage, bone, synovium, and vascular compartments in a single multi-tissue device. This holistic approach enables the study of inter-tissue crosstalk and systemic drug effects on the complete articular joint unit.

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Joint-on-Chip — image coming soon

Related Publications

Modular de- and re-construction of vascularized osteochondral tissues in an Organ-on-Chip dual-compartment platform

Mainardi, A., Barbero, A., Ehrbar, M., Rasponi, M., Martin, I. et al.

Journal of Orthopaedic Translation2025DOI

A Compartmentalized Joint-on-chip (JoC) Model to Unravel the Contribution of Cartilage and Synovium to Osteoarthritis Pathogenesis

Palma, C., Salehi, S., Polidoro, M.A., Moretti, M., Rasponi, M. et al.

Advanced Science2025DOI

An Organ-on-Chip Platform for Strain-Controlled, Tissue-Specific Compression of Cartilage and Mineralized Osteochondral Interface to Study Mechanical Overloading in Osteoarthritis

Mainardi, A., Börsch, A., Occhetta, P., Ivanek, R., Ehrbar, M. et al.

Advanced Healthcare Materials2025DOI

An Advanced Mechanically Active Osteoarthritis-on-Chip Model to Test Injectable Therapeutic Formulations: The SYN321 Case Study

Palma, C., Piazza, S., Visone, R., Ringom, R., Bjorklund, U. et al.

Advanced Healthcare Materials2024DOI

Development of a Microfluidic Vascularized Osteochondral Model as a Drug Testing Platform for Osteoarthritis

Salehi, S., Brambilla, S., Rasponi, M., Lopa, S., Moretti, M.

Advanced Healthcare Materials2024DOI

Immunomodulatory biomimetic nanoparticles target articular cartilage trauma after systemic administration

Mancino, C., Pasto, A., De Rosa, E., Dolcetti, L., Rasponi, M. et al.

Heliyon2023DOI

Modeling In Vitro Osteoarthritis Phenotypes in a Vascularized Bone Model Based on a Bone-Marrow Derived Mesenchymal Cell Line and Endothelial Cells

Pirosa, A., Tankus, E.B., Mainardi, A., Occhetta, P., Dönges, L. et al.

International Journal of Molecular Sciences2021DOI

Recapitulating monocyte extravasation to the synovium in an organotypic microfluidic model of the articular joint

Mondadori, C., Palombella, S., Salehi, S., Talò, G., Visone, R. et al.

Biofabrication2021DOI

Hyperphysiological compression of articular cartilage induces an osteoarthritic phenotype in a cartilage-on-a-chip model

Occhetta, P., Mainardi, A., Votta, E., Vallmajo-Martin, Q., Ehrbar, M. et al.

Nature Biomedical Engineering2019DOI

Developmentally inspired programming of adult human mesenchymal stromal cells toward stable chondrogenesis

Occhetta, P., Pigeot, S., Rasponi, M., Dasen, B., Mehrkens, A. et al.

PNAS2018DOI

Mechanical Induction of Osteoarthritis Traits in a Cartilage-on-a-Chip Model

Mainardi, A., Occhetta, P., Rasponi, M.

Methods in Molecular Biology2021DOI

Chondrocyte Hypertrophy in Osteoarthritis: Mechanistic Studies and Models for the Identification of New Therapeutic Strategies

Chawla, S., Mainardi, A., Majumder, N., Dönges, L., Kumar, B. et al.

Cells2022DOI

Engineered nasal cartilage for the repair of osteoarthritic knee cartilage defects

Rua, L.A., Mumme, M., Manferdini, C., Darwiche, S., Khalil, A.M. et al.

Science Translational Medicine2021DOI

Blockage of bone morphogenetic protein signalling counteracts hypertrophy in a human osteoarthritic micro-cartilage model

Chawla, S., Berkelaar, M.H.M., Dasen, B., Halleux, C., Guth-Gundel, S. et al.

Journal of Cell Science2020DOI

Challenges Toward the Identification of Predictive Markers for Human Mesenchymal Stromal Cells Chondrogenic Potential

Stüdle, C., Occhetta, P., Geier, F., Mehrkens, A., Barbero, A. et al.

Stem Cells Translational Medicine2019DOI

Learn, simplify and implement: developmental re-engineering strategies for cartilage repair

Occhetta, P., Stüdle, C., Barbero, A., Martin, I.

Swiss Medical Weekly2016DOI

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